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· Introduction
· From PacMan to
  TaqMan
  - a computer game
  revisited

· The advantages of
  real-time TaqMan
  PCR over
  conventional
  quantitative PCR

· Applications in
  Veterinary Medicine

· Allelic discrimination
· Discussion
· The veterinarian and
  his relationship with
  the next-generation
  PCR technology

· Acknowledgements
· References


 

Roquade



References
1. Addie, D.D., and Jarrett, J.O. 1992a. Feline coronavirus antibodies in cats. Vet Rec 131, 202-3.

2. Addie, D.D., and Jarrett, O. 1992b. A study of naturally occurring feline coronavirus infections in kittens. Vet Rec 130, 133-7.

3. Alard, P., Matriano, J.A., Socarras, S., Ortega, M.A., and Streilein, J.W. 1995. Detection of donor-derived cells by polymerase chain reaction in neonatally tolerant mice. Microchimerism fails to predict tolerance. Transplantation 60, 1125-30.

4. Bachmann, M.H., Mathiason-Dubard, C., Learn, G.H., Rodrigo, A.G., Sodora, D.L., Mazzetti, P., Hoover, E.A., and Mullins, J.I. 1997. Genetic diversity of feline immunodeficiency virus: dual infection, recombination, and distinct evolutionary rates among envelope sequence clades. J Virol 71, 4241-53.

5. Balkwill, F.R., and Burke, F. 1989. The cytokine network. Immunol Today 10, 299-304.

6. Belgrader, P., Benett, W., Hadley, D., Long, G., Mariella, R., Jr., Milanovich, F., Nasarabadi, S., Nelson, W., Richards, J., and Stratton, P. 1998a. Rapid pathogen detection using a microchip PCR array instrument. Clin Chem 44, 2191-4.

7. Belgrader, P., Okuzumi, M., Pourahmadi, F., Borkholder, D.A., and Northrup, M.A. 2000. A microfluidic cartridge to prepare spores for PCR analysis. Biosens Bioelectron 14, 849-52.

8. Belgrader, P., Smith, J.K., Weedn, V.W., and Northrup, M.A. 1998b. Rapid PCR for identity testing using a battery-powered miniature thermal cycler. J Forensic Sci 43, 315-9.

9. Bendinelli, M., Pistello, M., Matteucci, D., Lombardi, S., Baldinotti, F., Bandecchi, P., Ghilarducci, R., Ceccherini-Nelli, L., Garzelli, C., Poli, A., and et al. 1993. Small animal model of AIDS and the feline immunodeficiency virus. Ad Exp Med Biol 335, 189-202.

10. Bernhards, J., Weitzel, B., Werner, M., Rimpler, M., and Georgii, A. 1992. A new histological embedding method by low-temperature polymerisation of methyl methacrylate allowing immuno- and enzyme histochemical studies on semi-thin sections of undecalcified bone marrow biopsies. Histochemistry 98, 145-54.

11. Casey, G., Lo-Hsueh, M., Lopez, M.E., Vogelstein, B., and Stanbridge, E.J. 1991. Growth suppression of human breast cancer cells by the introduction of a wild-type p53 gene. Oncogene 6, 1791-7.

12. Chiang, P.W., Song, W.J., Wu, K.Y., Korenberg, J.R., Fogel, E.J., Van Keuren, M.L., Lashkari, D., and Kurnit, D.M. 1996. Use of a fluorescent-PCR reaction to detect genomic sequence copy number and transcriptional abundance. Genome Res 6, 1013-26.

13. Church, R.J., Hand, N.M., Rex, M., and Scotting, P.J. 1997. Non-isotopic in situ hybridization to detect chick sox gene mRNA in plastic-embedded tissue. Histochem J 29, 625-9.

14. Clementi, M., Menzo, S., Manzin, A., and Bagnarelli, P. 1995. Quantitative molecular methods in virology. Arch Virol 140, 1523-39.

15. Crivello, J.F. (1997). In "The PCR Technique: Quantitative PCR" (J.W. Larrick, ed.), pp. 170-172. Eaton Publishing, Mountain View, CA.

16. Del Pozo, V., De Andres, B., Gallardo, S., Cardaba, B., De Arruda-Chaves, E., Cortegano, M.I., Jurado, A., Palomino, P., Oliva, H., Aguilera, B., Posada, M., and Lahoz, C. 1997. Cytokine mRNA Expression in lung tissue from toxic oil syndrome patients: a Th2 immunological mechanism. Toxicology 118, 61-70.

17. Desrosiers, R.C. 1990. The simian immunodeficiency viruses. Annu Rev Immunol 8, 557-78.

18. Elbeik, T., Charlebois, E., Nassos, P., Kahn, J., Hecht, F.M., Yajko, D., Ng, V., and Hadley, K. 2000. Quantitative and cost comparison of ultrasensitive human immunodeficiency virus type 1 RNA viral load assays: Bayer bDNA quantiplex versions 3.0 and 2.0 and Roche PCR Amplicor monitor version 1.5. J Clin Microbiol 38, 1113-20.

19. Fehr, D., Holznagel, E., Bolla, S., Hauser, B., Herrewegh, A.A., Horzinek, M.C., and Lutz, H. 1997. Placebo-controlled evaluation of a modified life virus vaccine against feline infectious peritonitis: safety and efficacy under field conditions. Vaccine 15, 1101-9.

20. Fischer, M., Gunthard, H.F., Opravil, M., Joos, B., Huber, W., Bisset, L.R., Ott, P., Boni, J., Weber, R., and Cone, R.W. 2000. Residual HIV-RNA levels persist for up to 2.5 years in peripheral blood mononuclear cells of patients on potent antiretroviral therapy. AIDS Res Hum Retroviruses 16, 1135-40.

21. Foley, J.E., Poland, A., Carlson, J., and Pedersen, N.C. 1997. Patterns of feline coronavirus infection and fecal shedding from cats in multiple-cat environments. J Am Vet Med Assoc 210, 1307-12.

22. Gardner, M.B., and Luciw, P.A. 1989. Animal models of AIDS. FASEB Journal 3, 2593-606.

23. Gerardi, A.S. 1996. Bovine leucocyte adhesion deficiency: a review of a modern disease and its implications. Res Vet Sci 61, 183-6.

24. Gerber, J. 1995. Overview of the development of a midified live temperature-sensitive FIP virus vaccine. Feline Practice 23, 62-66.

25. Gilliland, G., Perrin, S., Blanchard, K., and Bunn, H.F. 1990. Analysis of cytokine mRNA and DNA: detection and quantitation by competitive polymerase chain reaction. Proc Natl Sci Acad U S A 725-9.

26. Gut, M., Leutenegger, C.M., Huder, J.B., Pedersen, N.C., and Lutz, H. 1999. One-tube fluorogenic reverse transcription-polymerase chain reaction for the quantitation of feline coronaviruses. J Virol Methods 77, 37-46.

27. Herrewegh, A.A., de Groot, R.J., Cepica, A., Egberink, H.F., Horzinek, M.C., and Rottier, P.J. 1995. Detection of feline coronavirus RNA in feces, tissues, and body fluids of naturally infected cats by reverse transcriptase PCR. J Clin Microbiol 33, 684-9.

28. Hofmann-Lehmann, R., Swenerton, R.K., Liska, V., Leutenegger, C.M., Lutz, H., McClure, H.M., and Ruprecht, R.M. 2000. Sensitive and robust one-tube real-time reverse transcriptase-polymerase chain reaction to quantify SIV RNA load: comparison of one- versus two-enzyme systems. AIDS Res Hum Retroviruses 16, 1247-1257.

29. Ibrahim, M.S., Lofts, R.S., Jahrling, P.B., Henchal, E.A., Weedn, V.W., Northrup, M.A., and Belgrader, P. 1998. Real-time microchip PCR for detecting single-base differences in viral and human DNA. Anal Chem 70, 2013-7.

30. Jackson, D.P., Lewis, F.A., Taylor, G.R., Boylston, A.W., and Quirke, P. 1990. Tissue extraction of DNA and RNA and analysis by the polymerase chain reaction. J Clin Pathol 43, 499-504.

31. Lantz, O., and Bendelac, A. 1994. An invariant T cell receptor alpha chain is used by a unique subset of major histocompatibility complex class I-specific CD4+ and CD4-8- T cells in mice and humans. J Exp Med 180, 1097-106.

32. Leutenegger, C.M., Klein, D., Hofmann-Lehmann, R., Mislin, C., Hummel, U., Böni, J., Boretti, F., Guenzburg, W.H., Lutz, H. 1999a. Rapid feline immunodeficiency virus provirus quantitation by polymerase chain reaction using the TaqMan fluorogenic real-time detection system. J. Virol. Methods 78, 105-116.

33. Leutenegger, C.M., Pusterla, N., Mislin, C.N., Weber, R., and Lutz, H. 1999b. Molecular evidence of coinfection of ticks with Borrelia burgdorferi sensu lato and the human granulocytic ehrlichiosis agent in Switzerland. J Clin Microbiol 37, 3390-1.

34. Leutenegger, C.M., von Rechenberg, B., Huder, J.B., Zlinsky, K., Mislin, C., Akens, M.K., Auer, J., and Lutz, H. 1999c. Quantitative real-time PCR for equine cytokine mRNA in nondecalcified bone tissue embedded in methyl methacrylate. Calcif Tissue Int 65, 378-83.

35. Leutenegger, C.M., Boretti, F., Mislin, C., Flynn, J.N., Schroff, M., Habel, A., Junghans, C., Koenig-Merediz, S.A.,Sigrist, B., Aubert, A., Pedersen, N.C., Wittig, B., Lutz, H. 2000a. Immunization of cats against feline immunodeficiency virus infection using minimalistic immunogenic defined gene expression vector vaccines expressing FIV-gp140 alone or with feline IL-12, IL-16 or CpG. J Virol, in press.

36. Leutenegger, C.M., Higgins, J., Matthews, T.B., Tarantal, A.F., Luciw, P.A., Pedersen, N.C.,North, T.W. 2000b. Real-time TaqMan PCR as a specific and more sensitive alternative to the branched-chain DNA assay for quantitation of simian immunodeficiency virus RNA. AIDS Research and Human Retroviruses, in press.

37. Leutenegger, C.M., Alluwaimi, A.M., Smith, W.L., Perani, L., Cullor, J.S. 2000c. Quantitation of bovine cytokine mRNA in milk cells of healthy cattle by real-time TaqMan polymerase chain reaction. Vet Immunol Immunopathol, in press.

38. Lischer, C.J., Leutenegger, C.M., Braun, U., and Lutz, H. 2000. Diagnosis of Lyme disease in two cows by the detection of Borrelia burgdorferi DNA . Vet Rec 146, 497-9.

39. Livak, K.J., Marmaro, J., and Todd, J.A. 1995. Towards fully automated genome-wide polymorphism screening. Nat Genet 9, 341-2.

40. Loeb, K.R., Jerome, K.R., Goddard, J., Huang, M., Cent, A., and Corey, L. 2000. High-throughput quantitative analysis of hepatitis B virus DNA in serum using the TaqMan fluorogenic detection system. Hepatology 32, 626-629.

41. McArdle, F., Tennant, B., Bennett, M. 1995. Independent evaluation of a modified live feline infectious peritonitis virus vaccine under experimental conditions (University of Liverpool experience). Feline Practice 23, 67-71.

42. Mellors, J.W., Rinaldo, C.R., Jr., Gupta, P., White, R.M., Todd, J.A., and Kingsley, L.A. 1996. Prognosis in HIV-1 infection predicted by the quantity of virus in plasma. Science 272, 1167-70.

43. Mullis, K., Faloona, F., Scharf, S., Saiki, R., Horn, G., and Erlich, H. 1986. Specific enzymatic amplification of DNA in vitro: the polymerase chain reaction. Cold Spring Harb Symp Quant Biol 51, 263-73.

44. Mullis, K.B., and Faloona, F.A. 1987. Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction. Methods Enzymol 155, 335-50.

45. Nagano, M., and Kelly, P.A. 1994. Tissue distribution and regulation of rat prolactin receptor gene expression. Quantitative analysis by polymerase chain reaction. J Biol Chem 269, 13337-45.

46. Nigro, J.M., Baker, S.J., Preisinger, A.C., Jessup, J.M., Hostetter, R., Cleary, K., Bigner, S.H., Davidson, N., Baylin, S., Devilee, P., and et al. 1989. Mutations in the p53 gene occur in diverse human tumour types. Nature 342, 705-8.

47. Pang, J., Modlin, J., and Yolken, R. 1992. Use of modified nucleotides and uracil-DNA glycosylase (UNG) for the control of contamination in the PCR-based amplification of RNA. Mol Cel Probes 6, 251-6.

48. Pedersen, N.C., Ho, E.W., Brown, M.L., and Yamamoto, J.K. 1987. Isolation of a T-lymphotropic virus from domestic cats with an immunodeficiency-like syndrome. Science 235, 790-3.

49. Poland, A.M., Vennema, H., Foley, J.E., and Pedersen, N.C. 1996. Two related strains of feline infectious peritonitis virus isolated from immunocompromised cats infected with a feline enteric coronavirus. J Clin Microbiol 34, 3180-4.

50. Postorino Reeves, N. 1995. Vaccination against naturally occurring FIP in a single large cat shelter. Feline Practice 23, 82-91.

51. Pusterla, N., Huder, J.B., Leutenegger, C.M., Braun, U., Madigan, J.E., and Lutz, H. 1999a. Quantitative real-time PCR for detection of members of the Ehrlichia phagocytophila genogroup in host animals and Ixodes ricinus ticks. J Clin Microbiol 37, 1329-31.

52. Pusterla, N., Leutenegger, C.M., Chae, J.S., Lutz, H., Kimsey, R.B., Dumler, J.S., and Madigan, J.E. 1999b. Quantitative evaluation of ehrlichial burden in horses after experimental transmission of human granulocytic Ehrlichia agent by intravenous inoculation with infected leukocytes and by infected ticks. J Clin Microbiol 37, 4042-4.

53. Pusterla, N., Leutenegger, C.M., Sigrist, B., Chae, J., Lutz, H., and Madigan, J.E. 2000. Detection and quantitation of Ehrlichia risticii genomic DNA in infected horses and snails by real-time PCR. Vet Parasitol 90, 129-135.

54. Rosenwirth, B., ten Haaft, P., Bogers, W.M., Nieuwenhuis, I.G., Niphuis, H., Kuhn, E.M., Bischofberger, N., Heeney, J.L., and Uberla, K. 2000. Antiretroviral therapy during primary immunodeficiency virus infection can induce persistent suppression of virus load and protection from heterologous challenge in rhesus macaques. J Virol 74, 1704-11.

55. Saiki, R.K., Scharf, S., Faloona, F., Mullis, K.B., Horn, G.T., Erlich, H.A., and Arnheim, N. 1985. Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. Science 230, 1350-4.

56. Saito, K. 1998. Morphology and molecular pathology: detection of hepatitis C virus RNA sequences in stained sections by microscopy-directed selective extraction. Rinsho Byori - Jap J Clin Pathol 46, 49-55.

57. Scholte, G.H., Van Doorn, L.J., Quint, W.G., and Lindeman, J. 1997. Polymerase chain reaction for the detection of Helicobacter pylori in formaldehyde-sublimate fixed, paraffin-embedded fastric biopsies. Diag Mol Pathol 6, 238-43.

58. Scott, F., Corapi, W., Olsen, C. 1995. Independent evaluation of a modified live FIPV vaccine under experimental conditions (Cornell experience). Feline Practice 23, 74-76.

59. Slamon, D.J., Godolphin, W., Jones, L.A., Holt, J.A., Wong, S.G., Keith, D.E., Levin, W.J., Stuart, S.G., Udove, J., Ullrich, A., and et al. 1989. Studies of the HER-2/neu proto-oncogene in human breast and ovarian cancer. Science 244, 707-12.

60. Sodora, D.L., Lee, F., Dailey, P.J., and Marx, P.A. 1998. A genetic and viral load analysis of the simian immunodeficiency virus during the acute phase in macaques inoculated by the vaginal route. AIDS Res Hum Retroviruses 14, 171-81.

61. Souaze, F., Ntodou-Thome, A., Tran, C.Y., Rostene, W., and Forgez, P. 1996. Quantitative RT-PCR: limits and accuracy. Biotechniques 21, 280-5.

62. Suryanarayana, K., Wiltrout, T.A., Vasquez, G.M., Hirsch, V.M., and Lifson, J.D. 1998. Plasma SIV RNA viral load determination by real-time quantification of product generation in reverse transcriptase-polymerase chain reaction. AIDS Res Hum Retroviruses 14, 183-9.

63. Taoufik, Y., Lantz, O., Wallon, C., Charles, A., Dussaix, E., and Delfraissy, J.F. 1997. Human immunodeficiency virus gp120 inhibits interleukin-12 secretion by human monocytes: an indirect interleukin-10-mediated effect. Blood 89, 2842-8.

64.Uberla K, Stahl-Hennig C, Bottiger D, Matz-Rensing K, Kaup FJ, Li J, Haseltine WA, Fleckenstein B, Hunsmann G, Oberg B, et al. 1995. Animal model for the therapy of acquired immunodeficiency syndrome with reverse transcriptase inhibitors. Proc Natl Acad Sci U S A, 92, 8210-4.

65. Umlauf, S.W., Beverly, B., Kang, S.M., Brorson, K., Tran, A.C., and Schwartz, R.H. 1993. Molecular regulation of the IL-2 gene: rheostatic control of the immune system. Immunol Rev 133, 177-97.

66. Vennema, H., Poland, A., Foley, J., and Pedersen, N.C. 1998. Feline infectious peritonitis viruses arise by mutation from endemic feline enteric coronaviruses. Virology 243, 150-7.

67. Vilbek, S., and Paton, D.J. 2000. A RT-PCR assay for the rapid recognition of border disease virus. Vet Res 31, 437-45.

68. Wang, A.M., Doyle, M.V., and Mark, D.F. 1989. Quantitation of mRNA by the polymerase chain reaction. Proc Natl Acad Sci U S A 86, 9717-21.

69. Wicki, R., Sauter, P., Mettler, C.,Natsch, S., Enzler, T.,Pusterla, N., Kuhnert, P., Egli, G., Bernasconi, M., Lienhard, R., Lutz, H., Leutenegger, C.M. 2000. A swiss army survey in Switzerland to determine the prevalence of Francisella tularensis, members of the Erlichia phagocytophila genogroup, Borrelia burgdorferi sensu lato, and tick-borne encephalitis virus in ticks. Europ J Clin Microbiol Inf Dis. 19, 427-432.

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